Laser scanning confocal microscopy is a widely accepted imaging technique when it comes to matching these requirements, and the choice of suitable detectors is crucial for realizing the full potential of this approach 9. Indeed, it would be desirable that any microscope could cope well with all current and future probes, delivering high-resolution spatial and dynamic information. The plethora of fluorophores available today provides a wealth of opportunities for researchers but also poses a technological challenge. The development of increasingly sophisticated labeling strategies 5, 6 and the continuing emergence of new genetically encoded fluorescent probes 7, 8 enable the study of almost all known cellular processes even at low expression levels. The study of molecular function typically requires innovative approaches to probing the interactions among partners in the cellular context 1 with unprecedented detail 2 and in closer-to-physiological conditions 3, 4. The rule is used to take measurements from the drawing and/or map, and then measured against the bar using the recorded distance.The ability to observe cellular structures and probe biomolecular functions with fluorescence microscopy is one of the most powerful experimental assets in the life sciences. How are they used?Ī scale bar is essentially a measuring aid and device, and whilst it can be used independently, it is typically paired with a standard rule. Scale bars can be drawn in any imperial or metric unit, however this is most commonly influenced by the size of the subject needing to be scaled.įor example, a floor plan of a house would most commonly be measured in millimetres or inches, but a map that represents a much larger area would be measured in meters/feet or miles/kilometres, and the accompanying scale bars would match this. If for example the drawing is being plotted to a metric 1:100 scale, and the scale bar units are in meters, then each one of its segments will represent 1 meter and be 100 times smaller than the 1 meter real-world measurement.Įqually, scale bars can be drawn at a 1:1 scale, and using the relative units of measurement to match the drawing, simply be plotted to the required size to match the drawing. How are they calculated?Ī scale bar if often calculated at the time of when the drawing or map is complete, and before it is plotted (printed) to a set size. The one KEY and fixed factor is that they must be straight!. Graphically however, there tends to be a few key and standard methods of representation as shown below, however equally there can be a high level of creativity in the way that the measuring segments and units are shown. If the drawing is of a different scale, it requires a different scale bar to match.įor this reason and in terms of the units used as measurement, there are many variations. Scale bars are bespoke to the scale of the drawing or map they are assigned to, and can only be transferred between one drawing to another if both drawings are of the same ratio (scale).įor example a scale bar showing an imperial 1:8” scale, can only be used to measure a 1:8” drawing. This is particularly useful when measuring via a digital screen or mobile device, and when a plotter is not available to print the required paper size. This is because the scale bar, no matter how big or small the drawing becomes, maintains its proportion to the drawings subject. Why are they important?Īside from making map and drawing reading easier, when a scale bar is present, the drawing or map doesn’t necessarily need to be printed to a specific size. The addition of a scale bar however means that the drawing can be measured using a traditional ruler and/or via any other well proportioned object such as a credit card or posit.Īs for maps, these come in a variety of shapes and sizes that due to the large areas being shown, rarely represent a traditional drawing scale, and a scale bar is therefore the only way to accurately measure distance. Particularly within the design, architectural, and engineering professions, in many instances drawings are printed to an exact size and can therefore be measured via a scale rule. A scale bar is a linear graphic that is divided into equal segments and used to measure distances on drawings and/or maps that are produced to a set scale, but not necessarily printed to one.
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